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KMID : 0359319910310020223
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Korean Journal of Veterinary Research
1991 Volume.31 No. 2 p.223 ~ p.228
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A study on production of early pregnancy diagnostic kit in cattle
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Abstract
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Most progesterone enzyme immunoassays(EIA) are used liquid-phase double-antibody or single-antibody seperation. These methods consume considerable time and reagents because of the requirements for several washing and centrifugation steps involving the reactants.
Because of these several problems, we were prompted eo develop an effective enzyme-linked immunosorbent assay(ELISA) system that would be equal or superior to RIA for assay of progesterone.
The results were obtained as follows.
1. Cross reaction of the progesterone antiserum with other steroids determined was shown with progesterone(100%), 11α-deoxycorti-costerone(2.271%), but the other steroids were shown below 0.9%.
2. Standard curve for progesterone ELISA was shown available difference according to progesterone concentration from 0 to 1,000pg/㎖.
3. The lower limit of sensitivity was 0.2pg/well
4. Progesterone concentration was 1.6ng/㎖ for before parturition, and that was below 0.5ng/㎖ for after parturition.
This development enzyme-linked immunosorbent assay for progesterone can be detected pregnancy diagnosis in cattle, and also applicable to research on physiological function including such as reproductive disorders.
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